rat glur2  (Alomone Labs)

Journal: Frontiers in Neuroinformatics

Article Title: Maximum likelihood-based estimation of diffusion coefficient is quick and reliable method for analyzing estradiol actions on surface receptor movements

doi: 10.3389/fninf.2023.1005936

Figure Lengend Snippet: The effect of E2 treatment on the diffusion coefficient of GluR2-AMPAR molecules in the soma’s plasma membranes of dPC12, live-cell. The E2 treatments were carried out by the concentration of 100 pM (A,B) and 100 nM (C,D) . Both the mean square displacement (MSD) (A,C) and the maximum likelihood-based estimation (MLE) (B,D) methods were used for further analysis to obtain the diffusion coefficients from the recorded trajectories. The graphs represent the groups as mean and SD values. The probability values of significant differences calculated by Kolmogorov–Smirnov test (* p < 0.05) and the number of trajectories in each group are also shown.

Article Snippet: Before single-molecule imaging, dPC12 were incubated with ATTO 488-labeled antibodies directed against the extracellular N-terminal domain of rat GluR2 (1:100, Alomone Labs Cat #: AGC-005-AG) in dRPMI cell culture medium at 37°C for 6 min. During the measurement period of ATTO 488-GluR2-AMPAR, 20–30 image series were recorded.

Techniques: Diffusion-based Assay, Concentration Assay

Journal: Frontiers in Neuroinformatics

Article Title: Maximum likelihood-based estimation of diffusion coefficient is quick and reliable method for analyzing estradiol actions on surface receptor movements

doi: 10.3389/fninf.2023.1005936

Figure Lengend Snippet: The trajectories length distribution from GluR2-AMPAR molecules in the soma’s plasma membranes of dPC12, live-cell in control state and after administration of 100 nM E2.

Article Snippet: Before single-molecule imaging, dPC12 were incubated with ATTO 488-labeled antibodies directed against the extracellular N-terminal domain of rat GluR2 (1:100, Alomone Labs Cat #: AGC-005-AG) in dRPMI cell culture medium at 37°C for 6 min. During the measurement period of ATTO 488-GluR2-AMPAR, 20–30 image series were recorded.

Techniques: Control

Journal: Brain Pathology

Article Title: Glutamatergic receptor expression changes in the Alzheimer's disease hippocampus and entorhinal cortex

doi: 10.1111/bpa.13005

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: GluA2 , KLH‐conjugated linear peptide corresponding to the cytoplasmic domain of rat GluR2 , Millipore, Rabbit, AB1768, RRID:AB_ 2247874 , 1:200 , – , 1:100.

Techniques: Recombinant, Purification

Journal: Brain Pathology

Article Title: Glutamatergic receptor expression changes in the Alzheimer's disease hippocampus and entorhinal cortex

doi: 10.1111/bpa.13005

Figure Lengend Snippet: Western blot against human hippocampus homogenates probed with glutamatergic receptor subunit GluA2, GluN1 and GluN2A antibodies (A). The use of the GluN2A blocking peptide (BLP‐GC002) (B) and omission of the primary antibodies (C) resulted in a complete absence of immunoreactivity except for a small amount of background lipofuscin staining. Section were stained with goat anti‐rabbit Alexa Fluor 647 (B,b; C,b) and goat anti‐mouse Alexa Fluor 488 (C,c). Nuclei were counterstained with Hoechst dye (blue) (B,a; C,a). Scale bars B–C = 50 µm

Article Snippet: GluA2 , KLH‐conjugated linear peptide corresponding to the cytoplasmic domain of rat GluR2 , Millipore, Rabbit, AB1768, RRID:AB_ 2247874 , 1:200 , – , 1:100.

Techniques: Western Blot, Blocking Assay, Staining

Journal: Brain Pathology

Article Title: Glutamatergic receptor expression changes in the Alzheimer's disease hippocampus and entorhinal cortex

doi: 10.1111/bpa.13005

Figure Lengend Snippet: GluA2 expression in the hippocampus, subiculum, entorhinal cortex, and superior temporal gyrus in human control and Alzheimer's disease cases visualized by 3,3′‐diaminobenzidine‐peroxidase immunohistochemistry. Staining appeared relatively strong within the str. pyramidale of the CA regions (A–D), with an increase in expression within the stratum (str.) moleculare of the dentate gyrus in AD (E1,2). Arrows indicating localization to processes and around pyramidal neurons. CA, cornu ammonis; DG, dentate gyrus; ECx, entorhinal cortex; HP, hippocampus; STG, superior temporal gyrus; str. pyr, stratum pyramidale; str. rad, stratum radiatum; str. gran, stratum granulosum; Sub, subiculum. Scale bars: A1,2 = 1000 µm; B1–E1, B2‐E2 = 100 µm; F1–H1, F2–H2 = 400 µm

Article Snippet: GluA2 , KLH‐conjugated linear peptide corresponding to the cytoplasmic domain of rat GluR2 , Millipore, Rabbit, AB1768, RRID:AB_ 2247874 , 1:200 , – , 1:100.

Techniques: Expressing, Control, Immunohistochemistry, Staining

Journal: Brain Pathology

Article Title: Glutamatergic receptor expression changes in the Alzheimer's disease hippocampus and entorhinal cortex

doi: 10.1111/bpa.13005

Figure Lengend Snippet: GluA2 expression is altered in the dentate gyrus in Alzheimer's disease. Photomicrographs of representative regions of the CA1(A), CA2 (B), CA3 (C), and dentate gyrus (D) showing GluA2 (red) and GluA2 overlaid with NeuN (green) immunoreactivity for representative Alzheimer's disease and control cases. AD, Alzheimer's disease; CA, cornu ammonis; DG, dentate gyrus; str. ori, straum oriens; str. pyr, stratum pyramidale; str. rad, stratum radiatum; str. mol, stratum moleculare; str. gran, stratum granulosum. Scale bars A–C = 100 µm, D = 50 µm

Article Snippet: GluA2 , KLH‐conjugated linear peptide corresponding to the cytoplasmic domain of rat GluR2 , Millipore, Rabbit, AB1768, RRID:AB_ 2247874 , 1:200 , – , 1:100.

Techniques: Expressing, Control

Journal: Brain Pathology

Article Title: Glutamatergic receptor expression changes in the Alzheimer's disease hippocampus and entorhinal cortex

doi: 10.1111/bpa.13005

Figure Lengend Snippet: GluA2 expression in the subiculum, entorhinal cortex and superior temporal gyrus in human control and Alzheimer's disease cases. Photomicrographs of representative regions of the subiculum (A), entorhinal cortex (B), and superior temporal gyrus (C) showing GluA2 (red) and GluA2 overlaid with NeuN (green) immunoreactivity for representative Alzheimer's disease and control cases. AD, Alzheimer's disease; CA, cornu ammonis; DG, dentate gyrus; ECx, entorhinal cortex: STG, superior temporal gyrus. Scale bars A–C, E–G = 100 µm, D = 50 µm

Article Snippet: GluA2 , KLH‐conjugated linear peptide corresponding to the cytoplasmic domain of rat GluR2 , Millipore, Rabbit, AB1768, RRID:AB_ 2247874 , 1:200 , – , 1:100.

Techniques: Expressing, Control

Journal: Brain Pathology

Article Title: Glutamatergic receptor expression changes in the Alzheimer's disease hippocampus and entorhinal cortex

doi: 10.1111/bpa.13005

Figure Lengend Snippet: Quantification of GluA2 immunoreactivity within the CA1, CA2, CA3, dentate gyrus hippocampal subfields, subiculum, entorhinal cortex, and superior temporal gyrus in control and Alzheimer's disease groups. Data are expressed as mean with error bars representing SEM. The figure shows significant increases in GluA2 expression in the stratum moleculare (D) in AD cases (white circles; n = 5–6) compared to controls (black circles; n = 5–7; Unpaired Mann‐Whitney test). AD, Alzheimer's disease; CA, cornu ammonis; DG, dentate gyrus; str. ori, straum oriens; str. pyr, stratum pyramidale; str. rad, stratum radiatum; str. mol, stratum moleculare; str. gran, stratum granulosum

Article Snippet: GluA2 , KLH‐conjugated linear peptide corresponding to the cytoplasmic domain of rat GluR2 , Millipore, Rabbit, AB1768, RRID:AB_ 2247874 , 1:200 , – , 1:100.

Techniques: Control, Expressing, MANN-WHITNEY

Journal: Frontiers in Neuroscience

Article Title: Evidence of Synaptic and Neurochemical Remodeling in the Retina of Aging Degus

doi: 10.3389/fnins.2020.00161

Figure Lengend Snippet: List of primary antibodies used for immunohistochemistry and in Western blotting.

Article Snippet: GluA2 , Ms , IgG1 , Fusion protein amino acids 834–883 cytoplasmic C-terminus of rat GluA2/GluR2 , Immunoblot of membranes from adult rat brain and adult GluA2/GluR2 knockout and wild-type mouse hippocampi , 1:1000 , Clone L21/32; UC Davis/NIH NeuroMab , .

Techniques: Immunohistochemistry, Western Blot, Recombinant, Membrane, Knock-Out